It was found that the gel matrix converts intralayer pyrene-pyrene (Py-Py) packing in the pure pyrene gel to interlayer Py-Py packing, thus giving an opposite chirality. The study not only deepened our understanding of the supramolecular chirality transfer but also unveiled the effects of an inert gel matrix in regulating the chiroptical properties.Elastomers saturated with gas at high pressure suffer from cavity nucleation, inflation, and deflation upon rapid or explosive decompression. Although this process often results in undetectable changes in appearance, it causes internal damage, hampers functionality (e.g., permeability), and shortens lifetime. Here, we tag a model poly(ethyl acrylate) elastomer with π-extended anthracene-maleimide adducts that fluoresce upon network chain scission, and map in 3D the internal damage present after a cycle of gas saturation and rapid decompression. Interestingly, we observe that each cavity observable during decompression results in a damaged region, the shape of which reveals a fracture locus of randomly oriented penny-shape cracks (i.e., with a flower-like morphology) that contain crack arrest lines. Thus, cavity growth likely proceeds discontinuously (i.e., non-steadily) through the stable and unstable fracture of numerous 2D crack planes. This non-destructive methodology to visualize in 3D molecular damage in polymer networks is novel and serves to understand how fracture occurs under complex 3D loads, predict mechanical aging of pristine looking elastomers, and holds potential to optimize cavitation-resistance in soft materials.Achiral block copolymers can self-assemble into helical structures when confined inside a cylindrical nanopore. However, controlling the chirality and the number of strands of helices is challenging. We present our simulation results of the influence of a chiral patch added to the confining nanopore on the structures and chirality of helices self-assembled from achiral cylinder-forming diblock copolymers under the confinement. Our results indicate that, when the designed patch is of proper geometry, it can induce the formation of helical structures and exhibit good control over their chirality. The bottom surface of the patch can induce the formation of a characteristic local structure near and parallel to it. It is the characteristic local structure that directs the formation of helices and of their chirality consistent with that of the patch. A large patch angle or the top/bottom surface of a weakly selective pore promotes the formation of double-helices compared to single-helices by enlarging the pitch of the helices near the patch or through the entropic attraction of the top surface of the pore to the minority blocks.While almost any kind of face mask offers some protection against particles and pathogens of different sizes, the most efficient ones make use of a layered structure where one or more layers are electrically charged. These electret layers are essential for the efficient filtration of difficult-to-capture small particles, yet the exact nature of electrostatic capture with respect to the charge on both the particles and the electret fibres as well as the effect of the immediate environment remain unclear. Here, we explore in detail the electrostatic interactions between the surface of a single charged electret fibre and a model of the SARS-CoV-2 virus. Using Poisson-Boltzmann electrostatics coupled to a detailed spike protein charge regulation model, we show how pH and salt concentration drastically change both the scale and the sign of the interaction. Furthermore, the configuration of the few spike proteins closest to the electret fibre turns out to be as important for the strength of the interaction as their total number on the virus envelope, a direct consequence of spike protein charge regulation. The results of our work elucidate the details of virus electrostatics and contribute to the general understanding of efficient virus filtration mechanisms.Plasma membrane (PM), a fundamental building component of a cell, is responsible for a variety of cell functions and biological processes. However, it is still challenging to acquire its morphology and morphological variation information via an effective approach. Herein, we report a PM imaging study regarding an aggregation-induced emission luminogen (AIEgen) called tetraphenylethylene-naphthalimide+ (TPE-NIM+), which is derived from our previously reported tetraphenylethylene-naphthalimide (TPE-NIM). The designed AIEgen (TPE-NIM+) shows significant characteristics of ultrafast staining, high photostability, wash-free property, and long retention time at the PM, which can structurally be correlated with its positively charged quaternary amine and hydrophobic moiety. TPE-NIM+ is further applied for staining of different cell lines, proving its universal PM imaging capability. Most importantly, we demonstrate that TPE-NIM+ can clearly delineate the contours of densely packed living cells with high cytocompatibility. Therefore, TPE-NIM+ as a PM imaging reagent superior to currently available commercial PM dyes shall find a number of applications in the biological/biomedical fields and even beyond.Anomalous diffusion in crowded and complex environments is widely studied due to its importance in intracellular transport, fluid rheology and materials engineering. Specifically, diffusion through the cytoskeleton, a network comprised of semiflexible actin filaments and rigid microtubules that interact both sterically and via crosslinking, plays a principal role in viral infection, vesicle transport and targeted drug delivery. Here, we elucidate the impact of crosslinking on particle diffusion in composites of actin and microtubules with actin-actin, microtubule-microtubule and actin-microtubule crosslinking. We analyze a suite of transport metrics by coupling single-particle tracking and differential dynamic microscopy. Using these complementary techniques, we find that particles display non-Gaussian and non-ergodic subdiffusion that is markedly enhanced by cytoskeletal crosslinking, which we attribute to suppressed microtubule mobility. https://www.selleckchem.com/products/fluorofurimazine.html However, the extent to which transport deviates from normal Brownian diffusion depends strongly on the crosslinking motif - with actin-microtubule crosslinking inducing the most pronounced anomalous characteristics.